LSBio
Complement C4d Antibody (clone 10-11)

Complement C4d Antibody (clone 10-11)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human Complement C4d antibodies →
$940.00each
Catalog Number: LS-C27178-0.1

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Overview

Description
Complement C4d antibody LS-C27178 is an unconjugated mouse monoclonal antibody to human Complement C4d. Validated for ELISA, IF, IHC and WB.
Application
ELISA, IF, IHC, IHC-F, IHC-P, WB
Reactivity
Hu
Nonreactive Species
Bovine, Cat, Dog, Guinea Pig, Mouse, Rabbit, Rat, Sheep
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IF/ICC
1:250 - 1:600
Recommended Dilution: IHC-F
1:100 - 1:750

Target

Target
Human Complement C4d
Antigen Species
Human
Epitope
Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a aoefootprinta of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
10-11
Isotype
IgG1

Immunogen

Immunogen
Native, from human plasma.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
BBS, 0.09% Sodium Azide
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
bbs

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.