LSBio
Collagen VII Antibody (C-Terminus, clone LH7.2)

Collagen VII Antibody (C-Terminus, clone LH7.2)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Western Blot
Reactivity:Guinea Pig, Human, Sheep
Format:Liquid
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$966.00each
Catalog Number: LS-C79667-100

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Overview

Description
Collagen VII antibody LS-C79667 is an unconjugated mouse monoclonal antibody to Collagen VII (C-Terminus) from human. It is reactive with human, guinea pig and sheep. Validated for ELISA, ICC, IF, IHC and WB.
Application
ELISA, ICC, IF, IHC, IHC-F, WB
Reactivity
GP, Hu, Sh
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IF/ICC
1:1000
Recommended Dilution: IHC-F
1:2000

Target

Target
Human Collagen VII
Antigen Species
Human
Epitope
Recognizes human tissue Type VII collagen. Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e., the non-helical carboxy terminal region of the Type VII collagen dimer. It is also reactive in immunoblotting against intact tissue Type VII collagen (250 kDa), but once the terminal regions are completely digested by pepsin, this reactivity is completely lost. No reactivity against collagen types I, II, III, IV, V, pepsin-digested collagen type VII, laminin and fibronectin. Species Crossreactivity: Basement membrane zone of stratified squamous epithelia of human, bovine, porcine, goat, guinea pig, monkey, marmoset and sheep.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
LH7.2
Isotype
IgG1

Immunogen

Immunogen
Full length native protein (purified) (Human). Insoluble fractions prepared from neonatal foreskin epidermal cells. Epitope: Recognizes an epitope located on collagenase digested Type VII collagen (150kD), i. e. the non-helical C-Terminus of the Type VII collagen dimer.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
15 mM Sodium Azide
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.