Anti collagen V antibody showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Collagen V Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Bovine, Human, Mammal (Other)
Format:	Liquid

$311.00each

Catalog Number: LS-C745282-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Collagen V antibody LS-C745282 is an unconjugated rabbit polyclonal antibody to Collagen V from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Application: ELISA, IHC, IP, WB
Reactivity: Bov, Hu, Mml
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:5000 - 1:50000
Recommended Dilution: IHC: 1:50 - 1:200
Recommended Dilution: IP: 1:100
Recommended Dilution: WB: 1:1000 - 1:10000

Target

Target: Mammal Collagen V
Antigen Species: Mammal (Other)
Epitope: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Collagen Type V from human and bovine placenta.
Immunogen Type: Native Protein (Purified from Natural Source)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl