Immunofluorescence analysis of HeLa cells, using Collagen V alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
COL5A1 / Collagen V Alpha 1 Antibody (aa301-350)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$341.00each
Catalog Number: LS-C119460-50
Catalog Number: LS-C119460
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- Description
- Collagen V Alpha 1 antibody LS-C119460 is an unconjugated rabbit polyclonal antibody to human Collagen V Alpha 1 (COL5A1) (aa301-350). Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Collagen alpha-1(V) chain | Collagen, type V, alpha 1 | COL5A1 | Collagen alpha 1(v) | Collagen V | Alpha 1 type V collagen
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P20908
- NCBI GenBank
- NM_000093
- SwissProt
- P20908
Target
- Target
- Human COL5A1 / Collagen V Alpha 1
- Antigen Species
- Human
- Epitope
- Collagen V alpha1 Antibody detects endogenous levels of total Collagen V alpha1 protein.
- Gene Name
- COL5A1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Synthetic peptide from human Collagen V alpha1. (AA Range: 301-350)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150 mM NaCl
Extracellular cleavage of collagen XVII is essential for correct cutaneous basement membrane formation. Machiko Nishimura, Wataru Nishie, Yoshinori Shirafuji, Satoru Shinkuma, Ken Natsuga, Hideki Nakamura, Daisuke Sawamura, Keiji Iwatsuki, Hiroshi Shimizu. The Breast : official journal of the European Society of Mastology. 2016 Jan;25:328-39. PubMed: 26604146