Western blot analysis of extracts of various cell lines, using RBM14 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
COAA / RBM14 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748218-20
Catalog Number: LS-C748218
For bulk orders or custom formulations:
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- Description
- RBM14 antibody LS-C748218 is an unconjugated rabbit polyclonal antibody to RBM14 (COAA) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- COAA | Paraspeckle protein 2 | RBM14 | RNA-binding motif protein 14 | RNA-binding protein 14 | SIP | TMEM137 | Transmembrane protein 137 | PSP2 | SYT-interacting protein | RNA binding motif protein 14 | SYTIP1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q96PK6
- NCBI GenBank
- NM_006328 | NP_006319.1
- SwissProt
- Q96PK6
Target
- Target
- Human COAA / RBM14
- Antigen Species
- Human
- Gene Name
- RBM14|RBM14-RBM4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 500-600 of human RBM14 (NP_006319.1). AAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLSMSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSK
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS