The SW480 cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: COTL1 transfected 293T cell lysate The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: A549 cell lysate Lane 3.: U87-MG cell lysate Lane 4.: HeLa cell lysate
CLP / COTL1 Antibody (clone AT1D6)
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
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Request a QuoteOverview
- Description
- COTL1 antibody LS-C755431 is an unconjugated mouse monoclonal antibody to human COTL1 (CLP). Validated for ELISA, Flow and WB.
- Application
- ELISA, FC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CLP | Coactosin-like protein | COTL1
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q14019
- NCBI GenBank
- NM_021149 | NP_066972.1
- SwissProt
- Q14019
Target
- Target
- Human CLP / COTL1
- Antigen Species
- Human
- Gene Name
- COTL1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT1D6
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human COTL1 (1-142aa) purified from E. coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Recommended Storage Buffer
- PBS