Extracts of ALLN-pretreated Jurkat cells unstimulated (1, 2) or stimulated with TNF-alpha (3-7) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature.
CHUK / IKKA / IKK Alpha Antibody (phospho-Ser32/Ser36)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Chicken, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$504.00each
Catalog Number: LS-C53801-100
Catalog Number: LS-C53801
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- IKK Alpha antibody LS-C53801 is an unconjugated rabbit polyclonal antibody to IKK Alpha (CHUK / IKKA) (pSer32/Ser36) from human. It is reactive with human, mouse, rat and other species. Validated for WB.
- Application
- WB
- Reactivity
- Ck, Hu, Ms, Por, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CHUK | I-kappa-B kinase alpha | I-kappa-B kinase-alpha | Ikb kinase alpha | IKK-A | IKBKA | IKK1 | NFKBIKA | I-kappa-B kinase 1 | IkappaB kinase | IKK Alpha | IKK-alpha | IKKA | Transcription factor 16 | IkappaBalpha Kinase | IkB kinase alpha subunit | IKK-a kinase | Ikkalpha | TCF-16 | TCF16
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O15111
- NCBI GenBank
- NM_001278 | NP_001269.3
- SwissProt
- O15111
Target
- Target
- Human CHUK / IKKA / IKK Alpha
- Antigen Species
- Human
- Epitope
- Human IkappaBalpha. Mouse and pig (100% homologous), rat and chicken (93% homologous) IkappaBalpha have not been tested, but are expected to react
- Gene Name
- CHUK
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic phosphopeptide derived from the region of human IkappaBalpha that contains serines 32 and 36. The sequence is conserved in mouse and pig.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Dulbecco's PBS (without Mg2+, Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) as a carrier.
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS