CHRM2 / M2 Antibody (aa168-192, clone B8E5)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Western Blot |
| Reactivity: | Bovine, Dog, Guinea Pig, Horse, Human, Mouse, Non-Human Primate, Pig, Rat, Sheep |
| Format: | Liquid |
$880.00each
Catalog Number: LS-C124189-100
Catalog Number: LS-C124189
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- Description
- M2 antibody LS-C124189 is an unconjugated mouse monoclonal antibody to M2 (CHRM2) (aa168-192) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IF, IHC, IHC-F, WB
- Reactivity
- Bov, Can, GP, Hr, Hu, Ms, NHP, Por, Rt, Sh
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 7TM receptor | CHRM2 | HM2 | M2 muscarinic receptor | M2-mAChR | Muscarinic receptor M2 | Muscarinic M2 receptor
- Recommended Dilution: ELISA
- 1:50
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: IHC-F
- 1:50
- Recommended Dilution: WB
- 1:50
- UniProt Number
- P08172
- NCBI GenBank
- NM_000739 | NP_000730.1
- SwissProt
- P08172
Target
- Target
- Human CHRM2 / M2
- Antigen Species
- Human
- Epitope
- Recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. Species cross-reactivity: rat, mouse and guinea pig.
- Gene Name
- CHRM2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- B8E5
- Isotype
- IgG2a
Immunogen
- Immunogen
- Synthetic peptide corresponding to the second extracellular loop aa168-192, VRTVEDGECYIQFFSNAAVTFGTAI(C) of human muscarinic acetylcholine receptor M2. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Elephant, Bat, Rabbit (96%); Hamster, Opossum, Platypus (92%); Xenopus (88%); Stickleback, Pufferfish, Zebrafish (84%); Turkey, Chicken (80%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% BSA
- Concentration
- 0.1 mg/mL
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
- Recommended Storage Buffer
- PBS
Airway hyperresponsiveness induced by repeated esophageal infusion of HCl in guinea pigs. Cheng YM, Cao AL, Zheng JP, Wang HW, Sun YS, Liu CF, Zhang BB, Wang Y, Zhu SL, Wu DZ. American journal of respiratory cell and molecular biology. 2014;51:701-8. PubMed: 24828018