Immunofluorescence staining of Chi3l3 in RAW246.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Chi3l3 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Chil3 / Chi3l3 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$164.00each
Catalog Number: LS-C818490-20
Catalog Number: LS-C818490
For bulk orders or custom formulations:
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- Description
- Chi3l3 antibody LS-C818490 is an unconjugated rabbit polyclonal antibody to mouse Chi3l3 (Chil3). Validated for ELISA, ICC, IF and WB.
- Application
- ELISA, ICC, IF, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O35744
- NCBI GenBank
- NP_034022.2
- SwissProt
- O35744
Target
- Target
- Mouse Chil3 / Chi3l3
- Antigen Species
- Mouse
- Epitope
- Mouse YM1/Chitinase 3 Like 3
- Gene Name
- Chil3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Mouse
Immunogen
- Immunogen
- Recombinant Mouse Chitinase-like 3 / Chi3l3 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS