Anti-Chk2 pT68 Antibody - Western Blot. Western blot of Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kD corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
CHEK2 / CHK2 Antibody (phospho-Thr68)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C19074-100
Catalog Number: LS-C19074
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- Description
- CHK2 antibody LS-C19074 is an unconjugated rabbit polyclonal antibody to human CHK2 (CHEK2) (pThr68). Validated for ELISA, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHEK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
- Recommended Dilution: ELISA
- 1:10000 - 1:70000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:200 - 1:2000
- UniProt Number
- O96017
- NCBI GenBank
- NM_007194 | NP_009125.1
- SwissProt
- O96017
Target
- Target
- Human CHEK2 / CHK2
- Antigen Species
- Human
- Epitope
- Reactivity occurs against human CHK2 pT68 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one amino acid residues in this sequence (90% homology). Cross reactivity with CHK2 homologues from other sources has not been determined.
- Gene Name
- CHEK2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 50-75 of Human CHK2.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl