1:100 staining human glioma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHEK2 / CHK2 Antibody (phospho-Ser516)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800896-100
Catalog Number: LS-C800896
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CHK2 antibody LS-C800896 is an unconjugated rabbit polyclonal antibody to CHK2 (CHEK2) (pSer516) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHEK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- O96017
- NCBI GenBank
- NM_007194 | NP_009125.1
- SwissProt
- O96017
Target
- Target
- Human CHEK2 / CHK2
- Antigen Species
- Human
- Epitope
- Phospho-Chk2 (Ser516) Antibody detects endogenous levels of Chk2 only when phosphorylated at Serine 516.
- Gene Name
- CHEK2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human Chk2 around the phosphorylation site of Serine 516.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS