CHK1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 1 uL anti-CHK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CHK1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa.
CHEK1 / CHK1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C817896-100
Catalog Number: LS-C817896
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- Description
- CHK1 antibody LS-C817896 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1). Validated for ELISA, ICC, IF, IP and WB.
- Application
- ELISA, ICC, IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
- Recommended Dilution: ELISA
- 1:25000 - 1:50000
- Recommended Dilution: IF/ICC
- 1:1500 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- O14757
- NCBI GenBank
- NM_001274 | NP_001265.2
- SwissProt
- O14757
Target
- Target
- Human CHEK1 / CHK1
- Antigen Species
- Human
- Epitope
- Human CHK1
- Gene Name
- CHEK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human Chk1 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS