Western blot analysis of extracts from HeLa cells: Line1: Using Chk1 (Ab-280) Antibody; Line2: The same antibody preincubated with synthesized peptide.
CHEK1 / CHK1 Antibody (p280)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826165-100
Catalog Number: LS-C826165
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- Description
- CHK1 antibody LS-C826165 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1) (p280). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- O14757
- NCBI GenBank
- NM_001274 | NP_001265.2
- SwissProt
- O14757
Target
- Target
- Human CHEK1 / CHK1
- Antigen Species
- Human
- Epitope
- Chk1 (Ab-280) Antibody detects endogenous levels of total Chk1 protein.
- Gene Name
- CHEK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl