Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-CENPE Antibody - Immunofluorescence Microscopy. monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. Alexa Fluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
CENPE Antibody (clone 1H12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C59522-100
Catalog Number: LS-C59522
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CENPE antibody LS-C59522 is an unconjugated mouse monoclonal antibody to human CENPE. Validated for ELISA, IF, IP and WB.
- Application
- ELISA, IF, IHC, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CENP-E | Centromere protein E | Centromere protein E (312kD) | Kinesin-related protein CENPE | PPP1R61 | KIF10 | CENPE | Centromere protein E, 312kDa | Kinesin family member 10
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IF/ICC
- 1:500 - 1:2000
- Recommended Dilution: IP
- 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q02224
- NCBI GenBank
- NM_001813 | NP_001804.2
- SwissProt
- Q02224
Target
- Target
- Human CENPE
- Antigen Species
- Human
- Gene Name
- CENPE
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 1H12
- Isotype
- IgG1
Immunogen
- Immunogen
- This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
