Immunofluorescent analysis of Carcinoembryonic Antigen (CEA, green) in BxPC-3 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 0.3% BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a CEA monoclonal antibody at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a goat anti-mouse IgG Superclonal secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst nuclear stain. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.