Western blot analysis using p27Kip1 (Phospho-Ser10) Antibody: Line1: The extracts from A2780 cells; Line2: The extracts from COLO205 cells; Line3: The extracts from COLO205 cells using p27Kip1 (Phospho-Ser10) Antibody preincubated with synthesized peptide.
CDKN1B / p27 Kip1 Antibody (phospho-Ser10)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C826357-100
Catalog Number: LS-C826357
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- Description
- P27 Kip1 antibody LS-C826357 is an unconjugated rabbit polyclonal antibody to p27 Kip1 (CDKN1B) (pSer10) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
- Recommended Dilution: ELISA
- 1:10000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P46527
- NCBI GenBank
- NM_004064 | NP_004055.1
- SwissProt
- P46527
Target
- Target
- Human CDKN1B / p27 Kip1
- Antigen Species
- Human
- Epitope
- p27Kip1 (Phospho-Ser10) Antibody detects endogenous levels of p27Kip1 only when phosphorylated at Serine 10.
- Gene Name
- CDKN1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of Serine10 (N-G-SP-P-S).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl