Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
CDK4 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg MCF-7 Whole Cell Lysate. 4 uL anti-CDK4 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CDK4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.
CDK4 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815551-100
Catalog Number: LS-C815551
For bulk orders or custom formulations:
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- Description
- CDK4 antibody LS-C815551 is an unconjugated rabbit polyclonal antibody to human CDK4. Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDK4 | Cell division protein kinase 4 | Crk3 | Cyclin-dependent kinase 4 | CMM3 | p34/cdk4 | PSK-J3
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P11802
- NCBI GenBank
- NM_000075 | NP_000066.1
- SwissProt
- P11802
Target
- Target
- Human CDK4
- Antigen Species
- Human
- Epitope
- Human CDK4
- Gene Name
- CDK4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human CDK4
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
