Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: l-Ptase). These blots are identical to the control except that they were incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
CDK1 / CDC2 Antibody (phospho-Tyr15)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat, Xenopus |
| Format: | Liquid |
$479.00each
Catalog Number: LS-C183-100
Catalog Number: LS-C183
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- Description
- CDC2 antibody LS-C183 is an unconjugated rabbit polyclonal antibody to CDC2 (CDK1) (pTyr15) from rat. It is reactive with human, mouse, rat and other species. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt, Xen
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDC28A | CDC2 | CDK1 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR | p-Tyr15 cdc2
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P06493
- NCBI GenBank
- NM_001786 | NP_001777.1
- SwissProt
- P06493
Target
- Target
- Rat CDK1 / CDC2
- Antigen Species
- Rat
- Epitope
- Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the X-phosphatase treatment
- Gene Name
- CDK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr1 of rat cdc2.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/mL BSA, 50% Glycerol
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
- Recommended Storage Buffer
- 10 mM HEPES|150 mM NaCl