Mab anti-Human p34 Cdc2 antibody (clone POH-1) is shown to detect human p34 Cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p34 Cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5000. A doublet band corresponding to human p34 Cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34 Cdc2. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
CDK1 / CDC2 Antibody (clone POH-1)
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CDC2 antibody LS-C18772 is an unconjugated mouse monoclonal antibody to CDC2 (CDK1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Cited in 3 publications.
- Application
- ELISA, IHC, IHC-P, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CDC28A | CDC2 | CDK1 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P06493
- NCBI GenBank
- NM_001786 | NP_001777.1
- SwissProt
- P06493
Target
- Target
- Human CDK1 / CDC2
- Antigen Species
- Human
- Epitope
- Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
- Gene Name
- CDK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- POH-1
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.5 M NaCl
Distinct forms of human CDC2 identified by novel monoclonal antibodies. Luks J, Draetta G, Bartek J. European journal of biochemistry / FEBS. 1992;207:169-76.
PubMed: 1628647
Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. Ye XS, Fincher RR, Tang A, McNeal KK, Gygax SE, Wexler AN, Ryan KB, James SW, Osmani SA. The Journal of biological chemistry. 1997;272:33384-93.
PubMed: 9407133
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1. Palmer A, Gavin AC, Nebreda AR. The EMBO journal. 1998;17:5037-47.
PubMed: 9724639 · PMC: PMC1170831