Anti-cdc2 Cyclin Dependent Kinase was used to detect human p34cdc2by Western blot of untreated (Control) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1 mM, 6.2 mM, 12.5 mM and 25.0 mM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased.
CDK1 / CDC2 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18771-100
Catalog Number: LS-C18771
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- Description
- CDC2 antibody LS-C18771 is an unconjugated rabbit polyclonal antibody to CDC2 (CDK1) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IHC-P, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDC28A | CDC2 | CDK1 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR
- Recommended Dilution: ELISA
- 1:60000
- Recommended Dilution: IHC-P
- 1:200 - 1:1000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:250 - 1:5000
- UniProt Number
- P06493
- NCBI GenBank
- NM_001786 | NP_001777.1
- SwissProt
- P06493
Target
- Target
- Human CDK1 / CDC2
- Antigen Species
- Human
- Epitope
- Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk2 from other species may also occur.
- Gene Name
- CDK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- CDC2 peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.01% Sodium Azide
- Concentration
- 85 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.