IHC analysis of P cadherin using anti-P cadherin antibody. P cadherin was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-P cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH3 / P-Cadherin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756577-100
Catalog Number: LS-C756577
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- Description
- P-Cadherin antibody LS-C756577 is an unconjugated rabbit polyclonal antibody to P-Cadherin (CDH3) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDH3 | Cadherin-3 | HJMD | P-cadherin | CDHP | PCAD | Placental cadherin
- Recommended Dilution: ELISA
- 0.1 - 0.5 ug/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P22223
- NCBI GenBank
- NM_001793 | NP_001784.2
- SwissProt
- P22223
Target
- Target
- Human CDH3 / P-Cadherin
- Antigen Species
- Human
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- CDH3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived human P cadherin recombinant protein (Position: Q126-H336).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl