IHC analysis of P cadherin using anti-P cadherin antibody. P cadherin was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-P cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH3 / P-Cadherin Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Lyophilized

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$480.00each

Catalog Number: LS-C756577-100

Unit Size

Quantity

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Overview

Description: P-Cadherin antibody LS-C756577 is an unconjugated rabbit polyclonal antibody to P-Cadherin (CDH3) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application: ELISA, IHC-P, WB
Reactivity: Hu, Ms, Rt
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CDH3 | Cadherin-3 | HJMD | P-cadherin | CDHP | PCAD | Placental cadherin
Recommended Dilution: ELISA: 0.1 - 0.5 ug/ml
Recommended Dilution: IHC-P: 0.5 - 1 µg/ml
Recommended Dilution: WB: 0.1 - 0.5 µg/ml
UniProt Number: P22223
NCBI GenBank: NM_001793, NP_001784.2
SwissProt: P22223

Target

Target: Human CDH3 / P-Cadherin
Antigen Species: Hu
Epitope: No cross reactivity with other proteins.
Gene Name: CDH3

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG
Origin Species: Hu

Immunogen

Immunogen: E. coli-derived human P cadherin recombinant protein (Position: Q126-H336).
Immunogen Type: Recom

Format

Conjugate/Label: Unconj
Format: Lyophilized
Formulation: Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative: Yes
Purification Method: AP

Storage & Handling

Storage Conditions: After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer: NaCl