Immunofluorescence staining of E-Cad in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human E-Cad polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
CDH1 / E Cadherin Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$212.00each
Catalog Number: LS-C817968-50
Catalog Number: LS-C817968
For bulk orders or custom formulations:
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- Description
- E Cadherin antibody LS-C817968 is an unconjugated rabbit polyclonal antibody to human E Cadherin (CDH1). Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:2500 - 1:10000
- UniProt Number
- P12830
- NCBI GenBank
- NM_004360 | NP_004351.1
- SwissProt
- P12830
Target
- Target
- Human CDH1 / E Cadherin
- Antigen Species
- Human
- Epitope
- Human E-cadherin
- Gene Name
- CDH1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Human E-Cadherin/CDH1/E-cad/CD324 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 50 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS