1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

CDC25C Antibody (phospho-Ser216)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$288.00each

Catalog Number: LS-C808495-100

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Quantity

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Overview

Description: CDC25C antibody LS-C808495 is an unconjugated rabbit polyclonal antibody to CDC25C (pSer216) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CDC25 | CDC25C phosphatase | CDC25C | CDC25Hu3 | Cell division cycle 25C | Phosphotyrosine phosphatase | PPP1R60 | M-phase inducer phosphatase 3 | Mitosis inducer CDC25
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: IHC: 1:50 - 1:200
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P30307
NCBI GenBank: NM_001790 | NP_001781.2
SwissProt: P30307

Target

Target: Human CDC25C
Antigen Species: Human
Epitope: Phospho-CDC25C (Ser216) Antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.
Gene Name: CDC25C

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: A synthesized peptide derived from human CDC25C around the phosphorylation site of Serine 216.
Immunogen Type: Synthetic Peptide (Linear)