LSBio
CDC25B Antibody (phospho-Ser323)

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody.

CDC25B Antibody (phospho-Ser323)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human CDC25B antibodies →
$365.00each
Catalog Number: LS-C825824-100

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Overview

Description
CDC25B antibody LS-C825824 is an unconjugated rabbit polyclonal antibody to CDC25B (pSer323) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDC25B | CDC25B phosphatase | Cell division cycle 25B | M-phase inducer phosphatase 2 | CDC25HU2
Recommended Dilution: ELISA
1:20000
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P30305
SwissProt
P30305

Target

Target
Human CDC25B
Antigen Species
Human
Epitope
CDC25B (Phospho-Ser323) antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323. (Human: Ser323; Mouse: Ser321; Rat: Ser319)
Gene Name
CDC25B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthesized phosphopeptide derived from human CDC25B around the phosphorylation site of serine 323 (S-P-SP-M-P).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS|150mM NaCl

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