LSBio
CDC25A Antibody (phospho-Thr507)

Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

CDC25A Antibody (phospho-Thr507)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human CDC25A antibodies →
$401.00each
Catalog Number: LS-C97430-400

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Overview

Description
CDC25A antibody LS-C97430 is an unconjugated rabbit polyclonal antibody to human CDC25A (pThr507). Validated for IF, IHC and WB. Cited in 1 publication.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDC25A | CDC25A2-CAG isoform | Cell division cycle 25A | CDC25A phosphatase | CDC25Hu1 | M-phase inducer phosphatase 1 | CDC25A2
Recommended Dilution: IHC-P
1:50 - 1:100
Recommended Dilution: WB
1:1000
UniProt Number
P30304
SwissProt
P30304

Target

Target
Human CDC25A
Antigen Species
Human
Epitope
This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A.
Gene Name
CDC25A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS
Pro-apoptotic role of Cdc25A: activation of cyclin B1/Cdc2 by the Cdc25A C-terminal domain. Chou ST, Yen YC, Lee CM, Chen MS. The Journal of biological chemistry. 2010;285:17833-45. PubMed: 20368335 PMC: PMC2878547

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