Immunofluorescence staining of mCD8A-His in Mouse Spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-MOUSE mCD8A-His polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
CD8A / CD8 Alpha Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Mouse |
| Format: | Liquid |
$164.00each
Catalog Number: LS-C818477-20
Catalog Number: LS-C818477
For bulk orders or custom formulations:
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- Description
- CD8 Alpha antibody LS-C818477 is an unconjugated rabbit polyclonal antibody to mouse CD8 Alpha (CD8A). Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC, IHC-P
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD8a molecule | CD8a antigen | CD8A | OKT8 T-cell antigen | Leu2 T-lymphocyte antigen | T8 T-cell antigen | T cell co-receptor | p32 | CD8 | Leu2 | T-cell antigen Leu2
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- P01732
- NCBI GenBank
- NM_001768 | NP_001759.3
- SwissProt
- P01732
Target
- Target
- Mouse CD8A / CD8 Alpha
- Antigen Species
- Mouse
- Epitope
- Mouse CD8 alpha
- Gene Name
- CD8A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse CD8 / CD8 alpha / Leu-2 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS