CD45 / LCA Antibody (clone YW62.3)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Frozen, Immunoprecipitation |
| Reactivity: | Mouse |
| Format: | Liquid |
$392.00each
Catalog Number: LS-C43233-0.25
Catalog Number: LS-C43233
For bulk orders or custom formulations:
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- Description
- LCA antibody LS-C43233 is an unconjugated rat monoclonal antibody to mouse LCA (CD45). Validated for Flow, IF, IHC and IP.
- Application
- FC, IF, IHC-F, IP
- Reactivity
- Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
- Recommended Dilution: FC
- 1:100 - 1:200
- UniProt Number
- P08575
- NCBI GenBank
- NM_002838, NP_002829.3
- SwissProt
- P08575
Target
- Target
- Mouse CD45 / LCA
- Antigen Species
- Ms
- Epitope
- Recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kD glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb;. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. Is reactive with all isoforms of murine CD45. N. B. Some reactivity with human tissue has been observed.
- Gene Name
- PTPRC
Antibody
- Host Species
- Rt
- Clonality
- Monoclonal
- Clone
- YW62.3
- Isotype
- IgG2b
Immunogen
- Immunogen
- Mouse spleen cells.
- Immunogen Type
- Cell
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS