LSBio
CD45 / LCA Antibody (clone IBL-3/16)

CD45 / LCA Antibody (clone IBL-3/16)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation
Reactivity:Mouse
Format:Liquid
See all Mouse CD45 / LCA antibodies →
$392.00each
Catalog Number: LS-C43542-0.25

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Overview

Description
LCA antibody LS-C43542 is an unconjugated rat monoclonal antibody to mouse LCA (CD45). Validated for Flow, IF, IHC and IP.
Application
FC, IF, IHC, IHC-F, IP
Reactivity
Ms
Nonreactive Species
Human, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
Recommended Dilution: FC
1:50 - 1:100
Recommended Dilution: IHC-F
1:50 - 1:100
UniProt Number
P08575
SwissProt
P08575

Target

Target
Mouse CD45 / LCA
Antigen Species
Mouse
Epitope
Recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kD single pass type I transmembrane glycoprotein belonging to the protein -tyrosine phosphatase family. CD45 has two domains and two domains. Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Is expected to recognize all isoforms of murine CD45. Has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques.
Gene Name
PTPRC

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
IBL-3/16
Isotype
IgG1

Immunogen

Immunogen
Purified B cells from mouse lymph nodes.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 0.1% BSA
Concentration
0.5 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.