IHC staining of FFPE human tonsil tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
CD45 / LCA Antibody (clone Bra55)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$379.00each
Catalog Number: LS-C782543-20
Catalog Number: LS-C782543
For bulk orders or custom formulations:
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- Description
- LCA antibody LS-C782543 is an unconjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow, IF and IHC.
- Application
- FC, IF, IHC
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA | PTPRC
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC
- 0.5 - 1 µg/ml
- UniProt Number
- P08575
- NCBI GenBank
- NM_002838 | NP_002829.3
- SwissProt
- P08575
Target
- Target
- Human CD45 / LCA
- Antigen Species
- Human
- Gene Name
- PTPRC
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- Bra55
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- REH cells (a non-T, non-B acute lymphoblastic leukaemia cell line) were used as the immunogen for the CD45 antibody.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
- Concentration
- 0.2 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 2-8°C.
- Recommended Storage Buffer
- 1X PBS