Western blot analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

CD44 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C331435-20

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: CD44 antibody LS-C331435 is an unconjugated rabbit polyclonal antibody to CD44 from human. It is reactive with human and mouse. Validated for IF, IP and WB.
Application: IF, IHC, IP, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CDW44 | CD44 | CD44 antigen | Cell surface glycoprotein CD44 | CSPG8 | ECMR-III | Epican | HUTCH-I | LHR | MDU2 | MC56 | MIC4 | Hermes antigen | Hyaluronate receptor | Phagocytic glycoprotein 1 | PGP-1 | PGP-I | Pgp1 | Phagocytic glycoprotein I | MDU3 | CD44R | HCELL | Heparan sulfate proteoglycan
Recommended Dilution: IF/ICC: 1:20 - 1:100
Recommended Dilution: IP: 1:20 - 1:100
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P16070
NCBI GenBank: NM_000610 | NP_000601.3
SwissProt: P16070

Target

Target: Human CD44
Antigen Species: Human
Epitope: human CD44
Gene Name: CD44

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-300 of human CD44 (NP_001001389.1). HVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATSTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTR
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)