IF staining of Daudi cells.

CD40 Antibody (CD40, clone 5D12)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Recombinant Monoclonal
Applications:	Blocking / Competition Assay, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Other
Reactivity:	Common Marmoset, Cynomolgus Monkey, Human
Format:	Liquid

$551.00each

Catalog Number: LS-C777451-0.2

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Description: This CD40 antibody is an unconjugated Rabbit/Human chimeric recombinant monoclonal antibody. Validated for Block, ELISA, Flo, IHC, IP, and SPR.
Application: Blocking, ELISA, FC, IHC, IP, Other
Reactivity: Cyno, Hu, Marm
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names: B-cell surface antigen CD40 | B cell-associated molecule | B cell surface antigen CD40 | CD40 | CD40 antigen | CD40 type II isoform | p50 | Bp50 | CD40L receptor | CDW40 | TNFRSF5
UniProt Number: P25942
NCBI GenBank: NM_001250 | NP_001241.1
SwissProt: P25942

Target: Human CD40
Antigen Species: Human
Epitope: ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis.
Gene Name: CD40

Immunogen: Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12.
Immunogen Type: Cell-Based Immunization