Western blot analysis of extracts of various cell lines, using CD300LF antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CD300LF / CD300f Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748854-20
Catalog Number: LS-C748854
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- CD300f antibody LS-C748854 is an unconjugated rabbit polyclonal antibody to human CD300f (CD300LF). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CD300f antigen | CLM-1 | CD300LF | CLM1 | CMRF35-like molecule 1 | Igsf13 | Inhibitory receptor IREM1 | NK inhibitory receptor | IREM-1 | NKIR | CD300f | IREM1
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q8TDQ1
- NCBI GenBank
- NP_620587.2
- SwissProt
- Q8TDQ1
Target
- Target
- Human CD300LF / CD300f
- Antigen Species
- Human
- Gene Name
- CD300LF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 23-150 of human CD300LF (NP_001276011.1). TQITGPTTVNGLERGSLTVQCVYRSGWETYLKWWCRGAIWRDCKILVKTSGSEQEVKRDRVSIKDNQKNRTFTVTMEDLMKTDADTYWCGIEKTGNDLGVTVQVTIDPAPVTQEETSSSPTLTGHHLD
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS