LSBio
CD3 Antibody (clone CD3-12, FITC)

CD3 Antibody (clone CD3-12, FITC)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Flow Cytometry
Reactivity:Human
Format:Liquid
See all Human CD3 antibodies →
$401.00each
Catalog Number: LS-C188140-0.1

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Overview

Description
CD3 antibody LS-C188140 is an FITC-conjugated rat monoclonal antibody to human CD3. Validated for Flow.
Application
FC
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:5 - 1:10

Target

Target
Human CD3
Antigen Species
Human
Epitope
Raised against a peptide representing an invariant cytoplasmic sequence within the CD3epsilon chain recognizes human CD3epsilon. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta;). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) ; ;. CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. Ashas been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross-reactivity for the CD3 marker. ;.

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
CD3-12
Isotype
IgG1

Immunogen

Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys). Percent identity by BLAST analysis: Chimpanzee, Gorilla, Ferret, Panda, Bovine, Dog, Cat, Opossum, Turkey, Chicken, Lizard (83%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 1% BSA
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.