LSBio
CD3 Antibody (clone 1F4)

CD3 Antibody (clone 1F4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Rat
Format:Liquid
See all Rat CD3 antibodies →
$296.00each
Catalog Number: LS-C58677-0.1

For bulk orders or custom formulations:

Request a Quote

Overview

Description
CD3 antibody LS-C58677 is an unconjugated mouse monoclonal antibody to rat CD3. Validated for Flow, IHC and IP.
Application
FC, IHC, IHC-F, IHC-P, IP
Reactivity
Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:10 - 1:25
Recommended Dilution: IHC-F
1:10 - 1:25
Recommended Dilution: IHC-P
1:10

Target

Target
Rat CD3
Antigen Species
Rat
Epitope
Recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
1F4
Isotype
IgM

Immunogen

Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.