Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using CD28 (Phospho-Tyr218) antibody.

CD28 Antibody (phospho-Tyr218)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$365.00each

Catalog Number: LS-C822226-100

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Description: CD28 antibody LS-C822226 is an unconjugated rabbit polyclonal antibody to CD28 (pTyr218) from human. It is reactive with human and mouse. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 | CD28 molecule
Recommended Dilution: ELISA: 1:40000
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: P10747
NCBI GenBank: NM_006139 | NP_006130.1
SwissProt: P10747

Target: Human CD28
Antigen Species: Human
Epitope: CD28 (Phospho-Tyr218) antibody detects endogenous levels of CD28 only when phosphorylated at tyrosine 218. (Human: Tyr218; Mouse: Tyr216)
Gene Name: CD28

Immunogen: Synthesized phosphopeptide derived from human CD28 around the phosphorylation site of tyrosine 218 (A-A-YP-R-S).
Immunogen Type: Synthetic Peptide (Linear)

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preservative: Yes
Purification Method: Affinity Purified