Fig-1: Cell surface Flow analysis of hCD28 in Jurkat cells using 0.5 ug/ 10^6 cells. Green represents isotype control;red represents anti-hCD28 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 30 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with Staining buffer).

CD28 Antibody (clone CB28)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry, Immunohistochemistry (general)
Reactivity:	Human
Format:	Liquid

$277.00each

Catalog Number: LS-C783427-25

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Overview

Description: CD28 antibody LS-C783427 is an unconjugated mouse monoclonal antibody to human CD28. Validated for Flow and IHC.
Application: FC, IHC
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 | CD28 molecule
Recommended Dilution: FC: 2 - 4 µg/10E6 cells
Recommended Dilution: IHC: 5 - 10 µg/ml
UniProt Number: P10747
NCBI GenBank: NM_006139 | NP_006130.1
SwissProt: P10747

Target

Target: Human CD28
Antigen Species: Human
Gene Name: CD28

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: CB28
Isotype: IgG1
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: A partial length recombinant protein from CD28
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS with 0.05% BSA and 0.05% sodium azide.
Concentration: 0.5 mg/mL
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C for up to 6 months. For long-term storage, store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS