CD274 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. 4uL anti-CD274 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD274 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 40 kDa.
CD274 / B7-H1 / PD-L1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation |
| Reactivity: | Cynomolgus Monkey |
| Format: | Liquid |
$212.00each
Catalog Number: LS-C817712-50
Catalog Number: LS-C817712
For bulk orders or custom formulations:
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- Description
- PD-L1 antibody LS-C817712 is an unconjugated rabbit polyclonal antibody to crab-eating macaque PD-L1 (CD274 / B7-H1). Validated for ELISA and IP.
- Application
- ELISA, IP
- Reactivity
- Cyno
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- UniProt Number
- Q9NZQ7
- NCBI GenBank
- NM_014143 | NP_054862.1
- SwissProt
- Q9NZQ7
Target
- Target
- Crab-eating macaque CD274 / B7-H1 / PD-L1
- Antigen Species
- Cynomolgus Monkey
- Epitope
- Cynomolgus PD-L1
- Gene Name
- CD274
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Cynomolgus PD-L1 / B7-H1 / CD274 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 50 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS