IHC testing of FFPE lung SCC with PD-L1 antibody (clone PDL1/2746). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
CD274 / B7-H1 / PD-L1 Antibody (clone PDL1/2746, Azide-free, BSA-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C782535-100
Catalog Number: LS-C782535
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- PD-L1 antibody LS-C782535 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow, IF, IHC and WB.
- Application
- FC, IF, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
- Recommended Dilution: FC
- 1 - 2 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 1 - 2 µg/ml
- Recommended Dilution: WB
- 1 - 2 µg/ml
- UniProt Number
- Q9NZQ7
- NCBI GenBank
- NM_014143 | NP_054862.1
- SwissProt
- Q9NZQ7
Target
- Target
- Human CD274 / B7-H1 / PD-L1
- Antigen Species
- Human
- Epitope
- This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
- Gene Name
- CD274
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- PDL1/2746
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- A portion of amino acids 39-191 from the human protein was used as the immunogen for this PD-L1 antibody.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1X PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Aliquot and store at -20°C.
- Recommended Storage Buffer
- 1X PBS