LSBio
CD247 / CD3 Zeta Antibody (phospho-Tyr142)

Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using CD3 ? (Phospho-Tyr142) antibody.

CD247 / CD3 Zeta Antibody (phospho-Tyr142)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human CD247 / CD3 Zeta antibodies →
$365.00each
Catalog Number: LS-C821821-100

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Overview

Description
CD3 Zeta antibody LS-C821821 is an unconjugated rabbit polyclonal antibody to CD3 Zeta (CD247) (pTyr142) from human. It is reactive with human and mouse. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD3 Zeta | CD3H | CD3Z | CD247 antigen, zeta subunit | CD247 molecule | CD3zeta chain | T3Z | TCR zeta chain | TCRZ | T-cell receptor T3 zeta chain | CD247 | CD247 antigen | CD3-ZETA | CD3Q
Recommended Dilution: ELISA
1:1000
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P20963
SwissProt
P20963

Target

Target
Human CD247 / CD3 Zeta
Antigen Species
Human
Epitope
CD3 Zeta (Phospho-Tyr142) antibody detects endogenous levels of CD3 Zeta only when phosphorylated at tyrosine 142. (Human: Tyr142; Mouse: Tyr143)
Gene Name
CD247

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Synthesized phosphopeptide derived from human CD3 Zeta around the phosphorylation site of tyrosine 142 (G-L-YP-Q-G).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS|150mM NaCl

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