Fig-1: Cell surface flow analysis of hCD20 on human PBMCs (Lymphocytes gated) using 0.5 µg/ 10^6 cells. Green represents isotype control; red represents anti-hCD20 antibody. Goat anti-mouse PE conjugated secondary antibody was used. (Cells were incubated with primary antibody for 45 min. then washed twice with FLOW Staining buffer by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with staining buffer).