LSBio
CD163 Antibody (clone ED2)

Immunohistochemistry of acetone fixed, cryostat sectioned rat spleen with Mouse anti-Rat CD163

CD163 Antibody (clone ED2)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Rat
Format:Liquid
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$384.00each
Catalog Number: LS-C188455-0.1

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Overview

Description
CD163 antibody LS-C188455 is an unconjugated mouse monoclonal antibody to rat CD163. Validated for IF, IHC, IP and WB.
Application
IF, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD163 antigen | CD163 | Macrophage-associated antigen | M130 | CD163 molecule | Hemoglobin scavenger receptor | MM130
Recommended Dilution: IHC-F
1:50 - 1:100
UniProt Number
Q86VB7
SwissProt
Q86VB7

Target

Target
Rat CD163
Antigen Species
Rt
Epitope
Recognizes the rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.* This clone may be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to ref. Whiteland et al.
Gene Name
CD163

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
ED2
Isotype
IgG1

Immunogen

Immunogen
Rat spleen cell homogenate.
Immunogen Type
Tiss-Extr

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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