Immunohistochemistry of acetone fixed, cryostat sectioned rat spleen with Mouse anti-Rat CD163

CD163 Antibody (clone ED2)

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Antibody:	Mouse Monoclonal
Applications:	Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Rat
Format:	Liquid

$384.00each

Catalog Number: LS-C188455-0.1

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Description: CD163 antibody LS-C188455 is an unconjugated mouse monoclonal antibody to rat CD163. Validated for IF, IHC, IP and WB.
Application: IF, IHC, IHC-F, IHC-P, IP, WB
Reactivity: Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CD163 antigen | CD163 | Macrophage-associated antigen | M130 | CD163 molecule | Hemoglobin scavenger receptor | MM130
Recommended Dilution: IHC-F: 1:50 - 1:100
UniProt Number: Q86VB7
NCBI GenBank: NM_004244 | NP_004235.4
SwissProt: Q86VB7

Target: Rat CD163
Antigen Species: Rat
Epitope: Recognizes the rat CD163 cell surface glycoprotein, a 175kD molecule also known as ED2. CD163 is expressed by approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.* This clone may be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde) - refer to ref. Whiteland et al.
Gene Name: CD163

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer: PBS