The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Cyclin H The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Jurkat cell lysate Lane 3.: Ramos cell lysate Lane 4.: Balb-3T3 cell lysate
CCNH / Cyclin H Antibody (clone AT3G6)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755419-50
Catalog Number: LS-C755419
For bulk orders or custom formulations:
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- Description
- Cyclin H antibody LS-C755419 is an unconjugated mouse monoclonal antibody to human Cyclin H (CCNH). Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Cyclin H | p34 | p37 | CAK | CAK complex subunit | CCNH | Cyclin-H | MO15-associated protein
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P51946
- NCBI GenBank
- NM_001239 | NP_001230.1
- SwissProt
- P51946
Target
- Target
- Human CCNH / Cyclin H
- Antigen Species
- Human
- Gene Name
- CCNH
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT3G6
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human Cyclin H (1-323aa) purified from E.coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Recommended Storage Buffer
- PBS