Western blot analysis of Mouse brain lysate showing detection of ~32.5 kDa Cyclin D3 protein using Rabbit Anti-Cyclin D3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D3 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~32.5 kDa. Other Band(s): ~45 kDa due to post translational modifications.
CCND3 / Cyclin D3 Antibody (FITC)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$482.00each
Catalog Number: LS-C774826-100
Catalog Number: LS-C774826
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- Description
- Cyclin D3 antibody LS-C774826 is an FITC-conjugated rabbit polyclonal antibody to Cyclin D3 (CCND3) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CCND3 | D3-type cyclin | G1/S-specific cyclin D3 | G1/S-specific cyclin-D3 | Cyclin D3
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P30281
- NCBI GenBank
- NM_001760 | NP_001751.1
- SwissProt
- P30281
Target
- Target
- Human CCND3 / Cyclin D3
- Antigen Species
- Human
- Epitope
- Detects 45 kDa, due to post translational modifications.
- Gene Name
- CCND3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human Cyclin D3 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS