Immunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.
CCND3 / Cyclin D3 Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745372-25
Catalog Number: LS-C745372
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- Description
- Cyclin D3 antibody LS-C745372 is an unconjugated rabbit polyclonal antibody to mouse Cyclin D3 (CCND3) (C-Terminus). Validated for ELISA, Flow, IHC, IP and WB.
- Application
- ELISA, FC, IHC, IP, WB
- Reactivity
- Ms
- Predicted Reactivity
- Bovine, Human
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CCND3 | D3-type cyclin | G1/S-specific cyclin D3 | G1/S-specific cyclin-D3 | Cyclin D3
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: FC
- 4 µg/ml
- Recommended Dilution: IHC
- 4 µg/ml
- Recommended Dilution: IP
- 5 µg/ml
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P30281
- NCBI GenBank
- NM_001760 | NP_001751.1
- SwissProt
- P30281
Target
- Target
- Mouse CCND3 / Cyclin D3
- Antigen Species
- Mouse
- Gene Name
- CCND3
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl