Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~33.1 kDa Cyclin D2 protein using Rabbit Anti-Cyclin D2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.1 kDa. Other Band(s): ~50 kDa due to post translational modifications.
CCND2 / Cyclin D2 Antibody (Atto 488)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$489.00each
Catalog Number: LS-C777040-100
Catalog Number: LS-C777040
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- Description
- Cyclin D2 antibody LS-C777040 is an Atto 488-conjugated rabbit polyclonal antibody to human Cyclin D2 (CCND2). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CCND2 | KIAK0002 | G1/S-specific cyclin D2 | G1/S-specific cyclin-D2 | Cyclin D2
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P30279
- NCBI GenBank
- NM_001759 | NP_001750.1
- SwissProt
- P30279
Target
- Target
- Human CCND2 / Cyclin D2
- Antigen Species
- Human
- Epitope
- Detects 33 kDa, ~50 kDa band is due to post translational modifications.
- Gene Name
- CCND2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the C-Terminus of Human Cyclin D2 (189-289 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Atto 488
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS