Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using Cyclin D1 (Phospho-Thr286) antibody.
CCND1 / Cyclin D1 Antibody (phospho-Thr286)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$365.00each
Catalog Number: LS-C821782-100
Catalog Number: LS-C821782
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- Description
- Cyclin D1 antibody LS-C821782 is an unconjugated rabbit polyclonal antibody to Cyclin D1 (CCND1) (pThr286) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | CCND1 | D11S287E | PRAD1 oncogene
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P24385
- NCBI GenBank
- NM_053056 | NP_444284.1
- SwissProt
- P24385
Target
- Target
- Human CCND1 / Cyclin D1
- Antigen Species
- Human
- Epitope
- Cyclin D1 (Phospho-Thr286) antibody detects endogenous levels of Cyclin D1 only when phosphorylated at threonine 286. (Human: Thr286; Mouse: Thr286)
- Gene Name
- CCND1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Synthesized phosphopeptide derived from human Cyclin D1 around the phosphorylation site of threonine 286 (A-C-TP-P-T).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year.
- Recommended Storage Buffer
- PBS|150mM NaCl