Western blot analysis of Rat brain lysate showing detection of ~33.7 kDa Cyclin D1 protein using Rabbit Anti-Cyclin D1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.7 kDa. Other Band(s): ~40 kDa due to post translational modifications.
CCND1 / Cyclin D1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Rat |
| Format: | Liquid |
$451.00each
Catalog Number: LS-C774691-100
Catalog Number: LS-C774691
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- Description
- Cyclin D1 antibody LS-C774691 is an unconjugated rabbit polyclonal antibody to Cyclin D1 (CCND1) from human. It is reactive with human and rat. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | CCND1 | D11S287E | PRAD1 oncogene
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P24385
- NCBI GenBank
- NM_053056 | NP_444284.1
- SwissProt
- P24385
Target
- Target
- Human CCND1 / Cyclin D1
- Antigen Species
- Human
- Epitope
- Detects 40 kDa, due to post translational modifications.
- Gene Name
- CCND1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the N-Terminus of Human Cyclin D1 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS