Immunofluorescence staining of CCNB2 in SW480 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CCNB2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
CCNB2 / Cyclin B2 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C815565-100
Catalog Number: LS-C815565
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- Description
- Cyclin B2 antibody LS-C815565 is an unconjugated rabbit polyclonal antibody to human Cyclin B2 (CCNB2). Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC-P
- Reactivity
- Hu
- Predicted Reactivity
- Cynomolgus Monkey
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Cyclin B2 | G2/mitotic-specific cyclin-B2 | HsT17299 | CCNB2
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- UniProt Number
- O95067
- NCBI GenBank
- NM_004701 | NP_004692.1
- SwissProt
- O95067
Target
- Target
- Human CCNB2 / Cyclin B2
- Antigen Species
- Human
- Epitope
- Human Cyclin B2
- Gene Name
- CCNB2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the N-Terminus of Human cyclin B2/CCNB2
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS