Western blot analysis using Anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kD corresponding to human Cyclin B1 (arrowhead). Approximately 50 ug of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10000 of HRP conjugated Goat-a-Rabbit IgG.
CCNB1 / Cyclin B1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C18811-100
Catalog Number: LS-C18811
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- Description
- Cyclin B1 antibody LS-C18811 is an unconjugated rabbit polyclonal antibody to Cyclin B1 (CCNB1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IB, IHC, IHC-P, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CCNB1 | CCNB | Cyclin B1 | G2/mitotic-specific cyclin-B1 | G2/mitotic-specific cyclin B1
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC-P
- 1:500
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P14635
- NCBI GenBank
- NM_031966 | NP_114172.1
- SwissProt
- P14635
Target
- Target
- Human CCNB1 / Cyclin B1
- Antigen Species
- Human
- Epitope
- Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin B1 from other species may also occur.
- Gene Name
- CCNB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.01% Sodium Azide
- Concentration
- 80 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.