IHC analysis of CCL6 using anti-CCL6 antibody. CCL6 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CCL6 antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Ccl6 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Mouse, Rat
Format:	Lyophilized

$480.00each

Catalog Number: LS-C756612-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Ccl6 antibody LS-C756612 is an unconjugated rabbit polyclonal antibody to Ccl6 from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Application: ELISA, IHC-P, WB
Reactivity: Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: C10 | Chemokine (C-C motif) ligand 6 | Ccl6 | MRP-1 | SCYA6
Recommended Dilution: ELISA: 1 - 5 ug/ml
Recommended Dilution: IHC-P: 0.5 - 1 µg/ml
Recommended Dilution: WB: 0.1 - 0.5 µg/ml
UniProt Number: P27784
NCBI GenBank: NM_009139 | NP_033165.1
SwissProt: P27784

Target

Target: Mouse Ccl6
Antigen Species: Mouse
Epitope: No cross reactivity with other proteins.
Gene Name: Ccl6

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Mouse

Immunogen

Immunogen: E. coli-derived mouse CCL6 recombinant protein (Position: G22-A116).
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Lyophilized
Formulation: Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Recommended Storage Buffer: NaCl